Blocking Assay:Article Title: METTL3-mediated m 6 A on nascent RNA coordinates translational and transcriptional programs to activate the NLRP3 inflammasome in macrophages.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IL-1β CST Cat# 12242S ASC CST Cat# 67824S NLRP3 CST Cat# 15101S β-ACTIN HUABIO Cat# ET1702-52 GAPDH HUABIO Cat# M1310-2 GSDMD Affinity Cat# AF4012 C/EBPβ Proteintech Cat# 23431-1-AP METTL3 CST Cat# 86132S IgG CST Cat# 2729 STAT3 HUABIO Cat# HM0617 p-STAT3 HUABIO Cat# HM0617 JAK1 Proteintech Cat# 66466-1-lg JAK2 HUABIO Cat# ET1607-35 JAK3 HUABIO Cat# ET1705-1 ZFP217 Abcam Cat# ab124927 METTL14 CST Cat# 48699 WTAP Proteintech Cat# 60188-L-lg YTHDF1 Proteintech Cat#66745-L-lg YTHDF2 Proteintech Cat#24744-L-AP YTHDF3 Proteintech Cat# 25537-1-AP FTO Abcam Cat# ab126605 ALKBH5 Proteintech Cat#16837-L-AP SC-35 Abcam Cat# ab11826 H3 HUABIO Cat#M1306-5 m 6 A Synaptic Systems Cat# 202 003 FITC-CD11b Biolegend Cat#557396 APC/Fire 750 -CD11c Biolegend Cat# 117351 PE-F4/80 Biolegend Cat# 565410 APC-CD45 Biolegend Cat#561018 PerCP/Cyanine5.5-CD206 Biolegend Cat#141715 Fc Block BD PharMingen Cat# 553141 Chemicals, peptides, and recombinant proteins Nuclease P1 NEB Cat# M0660S Alkaline phosphatase NEB Cat# M0525S D-glucose Aladdin Cat# 23403-54-5 Insulin Aladdin Cat# rp174360 Methylene blue SIGMA Cat# M9140 Actinomycin D MCE Cat# HY-17559 α-amanitin APExBIO Cat# A4548 Lipofectamine TM 3000 Thermofisher Cat# L3000015 RNase Inhibitor Thermofisher Cat# N8080119 Protease Inhibitor APExBIO Cat# K4002 Cycloheximide MCE Cat# HY-12320 Puromycin Solarbio Cat# P8230 (Continued on next page) 18 Cell Reports 45, 116808, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LPS SIGMA Cat# L2630-100MG ATP SIGMA Cat# A7699 FBS SIGMA Cat# F8318-500ML Opti-MEM TM Thermofisher Cat# 31985070 L-glutamine SIGMA Cat# G8540 MSU SIGMA Cat# U0881 NP-40 Thermofisher Cat# 28324 NaCl Sinopharm Cat# 10019308 Tris-HCl(pH = 7.0) Beyotime Cat# ST768 Sucrose SIGMA Cat# V900116 EDTA Invitrogen Cat# 15575-038 Triton X-100 SIGMA Cat# 9036-19-5 Glycerol Beyotime Cat# ST1353 DTT Beyotime Cat# ST043 Urea Macklin Cat# U820355 HEPES (pH = 7.5) Beyotime Cat# C0217 Nuclease-Free Water SIGMA Cat# W4502 Insulin Aladdin Cat# rp174360 High Fat Diet (60% cal/fat) XieTong Cat# D12492 Thinwall Polypropylene Tubes Beckman Coulter Cat# 331372 N6-Methyladenosine (Standard) MCE Cat# HY-N0086R Stattic MCE Cat# HY-13818 Critical Commercial Assays IL-1β ELISA Kit RayBio Cat# ELM-Il1β-1 IL-18 ELISA Kit HUABIO Cat# EM0034 TNFα ELISA Kit HUABIO Cat# EM0010 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat# 11402ES60 Cut-Tag kit Vazyme Cat# TD901 DNA Clean Beads Vazyme Cat# N411 Cut-Run-qPCR kit Vazyme Cat# HD101 and HD103 cDNA synthesis Kit Yeasen Cat# 1139ES60 qPCR SYBR Green Master Mix Yeasen Cat# 11201ES08 Click TM EU-488 RNA synthesis kit Beyotime Cat# R0301S miRNeasy Mini Kit QIAGEN Cat# 217004 GenElute TM mRNA Miniprep Kit SIGMA Cat# MRN70-1KT Fluorescent in Situ Hybridization Kit Genepharma Cat# F40171 Glucometer Yuwell NA Digital caliper Mitutoyo NA Deposited data Datebase: PRJNA1150098 This paper https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA1150098 Database: PRJNA1149668 This paper https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA1149668 Database: PRJNA1369820 This paper https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA1369820 Experimental Models: Cell Lines J774A.1 CTCC 400–0361 HEK293T ATCC CRL-11268 L929 Professor Qingqing Wang NA Experimental Models: mice Mouse: C57BL/6 Beijing Vital River Technology Co., Ltd NA (Continued on next page) Cell Reports 45, 116808, January 27, 2026 19
Recombinant:Article Title: METTL3-mediated m 6 A on nascent RNA coordinates translational and transcriptional programs to activate the NLRP3 inflammasome in macrophages.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IL-1β CST Cat# 12242S ASC CST Cat# 67824S NLRP3 CST Cat# 15101S β-ACTIN HUABIO Cat# ET1702-52 GAPDH HUABIO Cat# M1310-2 GSDMD Affinity Cat# AF4012 C/EBPβ Proteintech Cat# 23431-1-AP METTL3 CST Cat# 86132S IgG CST Cat# 2729 STAT3 HUABIO Cat# HM0617 p-STAT3 HUABIO Cat# HM0617 JAK1 Proteintech Cat# 66466-1-lg JAK2 HUABIO Cat# ET1607-35 JAK3 HUABIO Cat# ET1705-1 ZFP217 Abcam Cat# ab124927 METTL14 CST Cat# 48699 WTAP Proteintech Cat# 60188-L-lg YTHDF1 Proteintech Cat#66745-L-lg YTHDF2 Proteintech Cat#24744-L-AP YTHDF3 Proteintech Cat# 25537-1-AP FTO Abcam Cat# ab126605 ALKBH5 Proteintech Cat#16837-L-AP SC-35 Abcam Cat# ab11826 H3 HUABIO Cat#M1306-5 m 6 A Synaptic Systems Cat# 202 003 FITC-CD11b Biolegend Cat#557396 APC/Fire 750 -CD11c Biolegend Cat# 117351 PE-F4/80 Biolegend Cat# 565410 APC-CD45 Biolegend Cat#561018 PerCP/Cyanine5.5-CD206 Biolegend Cat#141715 Fc Block BD PharMingen Cat# 553141 Chemicals, peptides, and recombinant proteins Nuclease P1 NEB Cat# M0660S Alkaline phosphatase NEB Cat# M0525S D-glucose Aladdin Cat# 23403-54-5 Insulin Aladdin Cat# rp174360 Methylene blue SIGMA Cat# M9140 Actinomycin D MCE Cat# HY-17559 α-amanitin APExBIO Cat# A4548 Lipofectamine TM 3000 Thermofisher Cat# L3000015 RNase Inhibitor Thermofisher Cat# N8080119 Protease Inhibitor APExBIO Cat# K4002 Cycloheximide MCE Cat# HY-12320 Puromycin Solarbio Cat# P8230 (Continued on next page) 18 Cell Reports 45, 116808, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LPS SIGMA Cat# L2630-100MG ATP SIGMA Cat# A7699 FBS SIGMA Cat# F8318-500ML Opti-MEM TM Thermofisher Cat# 31985070 L-glutamine SIGMA Cat# G8540 MSU SIGMA Cat# U0881 NP-40 Thermofisher Cat# 28324 NaCl Sinopharm Cat# 10019308 Tris-HCl(pH = 7.0) Beyotime Cat# ST768 Sucrose SIGMA Cat# V900116 EDTA Invitrogen Cat# 15575-038 Triton X-100 SIGMA Cat# 9036-19-5 Glycerol Beyotime Cat# ST1353 DTT Beyotime Cat# ST043 Urea Macklin Cat# U820355 HEPES (pH = 7.5) Beyotime Cat# C0217 Nuclease-Free Water SIGMA Cat# W4502 Insulin Aladdin Cat# rp174360 High Fat Diet (60% cal/fat) XieTong Cat# D12492 Thinwall Polypropylene Tubes Beckman Coulter Cat# 331372 N6-Methyladenosine (Standard) MCE Cat# HY-N0086R Stattic MCE Cat# HY-13818 Critical Commercial Assays IL-1β ELISA Kit RayBio Cat# ELM-Il1β-1 IL-18 ELISA Kit HUABIO Cat# EM0034 TNFα ELISA Kit HUABIO Cat# EM0010 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat# 11402ES60 Cut-Tag kit Vazyme Cat# TD901 DNA Clean Beads Vazyme Cat# N411 Cut-Run-qPCR kit Vazyme Cat# HD101 and HD103 cDNA synthesis Kit Yeasen Cat# 1139ES60 qPCR SYBR Green Master Mix Yeasen Cat# 11201ES08 Click TM EU-488 RNA synthesis kit Beyotime Cat# R0301S miRNeasy Mini Kit QIAGEN Cat# 217004 GenElute TM mRNA Miniprep Kit SIGMA Cat# MRN70-1KT Fluorescent in Situ Hybridization Kit Genepharma Cat# F40171 Glucometer Yuwell NA Digital caliper Mitutoyo NA Deposited data Datebase: PRJNA1150098 This paper https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA1150098 Database: PRJNA1149668 This paper https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA1149668 Database: PRJNA1369820 This paper https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA1369820 Experimental Models: Cell Lines J774A.1 CTCC 400–0361 HEK293T ATCC CRL-11268 L929 Professor Qingqing Wang NA Experimental Models: mice Mouse: C57BL/6 Beijing Vital River Technology Co., Ltd NA (Continued on next page) Cell Reports 45, 116808, January 27, 2026 19
Protease Inhibitor:Article Title: METTL3-mediated m 6 A on nascent RNA coordinates translational and transcriptional programs to activate the NLRP3 inflammasome in macrophages.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IL-1β CST Cat# 12242S ASC CST Cat# 67824S NLRP3 CST Cat# 15101S β-ACTIN HUABIO Cat# ET1702-52 GAPDH HUABIO Cat# M1310-2 GSDMD Affinity Cat# AF4012 C/EBPβ Proteintech Cat# 23431-1-AP METTL3 CST Cat# 86132S IgG CST Cat# 2729 STAT3 HUABIO Cat# HM0617 p-STAT3 HUABIO Cat# HM0617 JAK1 Proteintech Cat# 66466-1-lg JAK2 HUABIO Cat# ET1607-35 JAK3 HUABIO Cat# ET1705-1 ZFP217 Abcam Cat# ab124927 METTL14 CST Cat# 48699 WTAP Proteintech Cat# 60188-L-lg YTHDF1 Proteintech Cat#66745-L-lg YTHDF2 Proteintech Cat#24744-L-AP YTHDF3 Proteintech Cat# 25537-1-AP FTO Abcam Cat# ab126605 ALKBH5 Proteintech Cat#16837-L-AP SC-35 Abcam Cat# ab11826 H3 HUABIO Cat#M1306-5 m 6 A Synaptic Systems Cat# 202 003 FITC-CD11b Biolegend Cat#557396 APC/Fire 750 -CD11c Biolegend Cat# 117351 PE-F4/80 Biolegend Cat# 565410 APC-CD45 Biolegend Cat#561018 PerCP/Cyanine5.5-CD206 Biolegend Cat#141715 Fc Block BD PharMingen Cat# 553141 Chemicals, peptides, and recombinant proteins Nuclease P1 NEB Cat# M0660S Alkaline phosphatase NEB Cat# M0525S D-glucose Aladdin Cat# 23403-54-5 Insulin Aladdin Cat# rp174360 Methylene blue SIGMA Cat# M9140 Actinomycin D MCE Cat# HY-17559 α-amanitin APExBIO Cat# A4548 Lipofectamine TM 3000 Thermofisher Cat# L3000015 RNase Inhibitor Thermofisher Cat# N8080119 Protease Inhibitor APExBIO Cat# K4002 Cycloheximide MCE Cat# HY-12320 Puromycin Solarbio Cat# P8230 (Continued on next page) 18 Cell Reports 45, 116808, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LPS SIGMA Cat# L2630-100MG ATP SIGMA Cat# A7699 FBS SIGMA Cat# F8318-500ML Opti-MEM TM Thermofisher Cat# 31985070 L-glutamine SIGMA Cat# G8540 MSU SIGMA Cat# U0881 NP-40 Thermofisher Cat# 28324 NaCl Sinopharm Cat# 10019308 Tris-HCl(pH = 7.0) Beyotime Cat# ST768 Sucrose SIGMA Cat# V900116 EDTA Invitrogen Cat# 15575-038 Triton X-100 SIGMA Cat# 9036-19-5 Glycerol Beyotime Cat# ST1353 DTT Beyotime Cat# ST043 Urea Macklin Cat# U820355 HEPES (pH = 7.5) Beyotime Cat# C0217 Nuclease-Free Water SIGMA Cat# W4502 Insulin Aladdin Cat# rp174360 High Fat Diet (60% cal/fat) XieTong Cat# D12492 Thinwall Polypropylene Tubes Beckman Coulter Cat# 331372 N6-Methyladenosine (Standard) MCE Cat# HY-N0086R Stattic MCE Cat# HY-13818 Critical Commercial Assays IL-1β ELISA Kit RayBio Cat# ELM-Il1β-1 IL-18 ELISA Kit HUABIO Cat# EM0034 TNFα ELISA Kit HUABIO Cat# EM0010 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat# 11402ES60 Cut-Tag kit Vazyme Cat# TD901 DNA Clean Beads Vazyme Cat# N411 Cut-Run-qPCR kit Vazyme Cat# HD101 and HD103 cDNA synthesis Kit Yeasen Cat# 1139ES60 qPCR SYBR Green Master Mix Yeasen Cat# 11201ES08 Click TM EU-488 RNA synthesis kit Beyotime Cat# R0301S miRNeasy Mini Kit QIAGEN Cat# 217004 GenElute TM mRNA Miniprep Kit SIGMA Cat# MRN70-1KT Fluorescent in Situ Hybridization Kit Genepharma Cat# F40171 Glucometer Yuwell NA Digital caliper Mitutoyo NA Deposited data Datebase: PRJNA1150098 This paper https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA1150098 Database: PRJNA1149668 This paper https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA1149668 Database: PRJNA1369820 This paper https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA1369820 Experimental Models: Cell Lines J774A.1 CTCC 400–0361 HEK293T ATCC CRL-11268 L929 Professor Qingqing Wang NA Experimental Models: mice Mouse: C57BL/6 Beijing Vital River Technology Co., Ltd NA (Continued on next page) Cell Reports 45, 116808, January 27, 2026 19
Pyrolysis Gas Chromatography:Article Title: WTAP regulates postnatal development of brown adipose tissue by stabilizing METTL3 in mice
Article Snippet: .. Data represent the mean ± SEM. n was the number of biologically independent mice. a b W ta p flo x/ flo x W ta pB K O W ta pB K O M et tl3 -B O E 0 2 4 6 F o o d i n ta k e ( g /m o u s e /d a y ) D ar k cy cl e Li gh t c yc le 0 50 100 150 200 250 P h y s ic a l a c ti v it y ( m ) Wtap flox/flox Wtap-BKO Wtap-BKO Mettl3-BOE ** * Wtap flox/flox Wtap-BKO Wtap-BKO Mettl3-BOE c 100um Supplementary Table 3 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies METTL3 CST Cat#96391 WTAP proteintech Cat# 10200-1-AP UCP1 SIGMA Cat#U6382 PGC-1α proteintech Cat#66369-1-lg β-Actin proteintech Cat#60008-1-lg PPARγ proteintech Cat#16643-1-AP PRDM16 Abclonal Cat# A11581 Total OXPHOS abcom Cat#ab110413 GAPDH proteintech Cat#60004-1-lg Flag SIGMA Cat# F1804 AKT CST Cat#9272 P-AKT(S473) CST Cat#9271 Chemicals IBMX(isobutylmethylx anthine) SIGMA Cat#I5879 T3 SIGMA Cat#T2877 ROSIGLITAZONE SIGMA Cat#R2408 INDOMETHACIN CRYSTALLINE SIGMA Cat#I7378 DEXAMETHASINE CRYSTALLINE SIGMA Cat#D1756 INSULIN SIGMA Cat#I5500 RIPA Buffer Solarbio Cat#R0020 Blue Plus II Protein Marker Transgen Biotech Cat#L50824 High Pure dNTPs Transgen Biotech Cat#20201025 GoTaq Green Master Mix Promega Cat#M7122 M-MLV reverse transcriptase Promega Cat#M1701 Random Primers Promega Cat#C1181 FBS TRINTY TEK Cat#01010102 Collagenase Type2 Worthington Cat#LS004176 SYBRTM Green Mix Roche Cat#4913914001 TriPure Isolation Reagent Roche Cat#94015120 Methanol Fisher Chemical Cat#A452 Water Fisher Chemical Cat#A4 Acetonitrile Fisher Chemical Cat#A998 Ammonium acetate Fisher Chemical Cat#A114 Ammonium hydroxide Fisher Chemical Cat#470 MG132 Sigma Cat#474787 leupetin Sigma Cat# L2884 Software Graphpad Graphpad softwave Graphpad.com ..
Marker:Article Title: WTAP regulates postnatal development of brown adipose tissue by stabilizing METTL3 in mice
Article Snippet: .. Data represent the mean ± SEM. n was the number of biologically independent mice. a b W ta p flo x/ flo x W ta pB K O W ta pB K O M et tl3 -B O E 0 2 4 6 F o o d i n ta k e ( g /m o u s e /d a y ) D ar k cy cl e Li gh t c yc le 0 50 100 150 200 250 P h y s ic a l a c ti v it y ( m ) Wtap flox/flox Wtap-BKO Wtap-BKO Mettl3-BOE ** * Wtap flox/flox Wtap-BKO Wtap-BKO Mettl3-BOE c 100um Supplementary Table 3 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies METTL3 CST Cat#96391 WTAP proteintech Cat# 10200-1-AP UCP1 SIGMA Cat#U6382 PGC-1α proteintech Cat#66369-1-lg β-Actin proteintech Cat#60008-1-lg PPARγ proteintech Cat#16643-1-AP PRDM16 Abclonal Cat# A11581 Total OXPHOS abcom Cat#ab110413 GAPDH proteintech Cat#60004-1-lg Flag SIGMA Cat# F1804 AKT CST Cat#9272 P-AKT(S473) CST Cat#9271 Chemicals IBMX(isobutylmethylx anthine) SIGMA Cat#I5879 T3 SIGMA Cat#T2877 ROSIGLITAZONE SIGMA Cat#R2408 INDOMETHACIN CRYSTALLINE SIGMA Cat#I7378 DEXAMETHASINE CRYSTALLINE SIGMA Cat#D1756 INSULIN SIGMA Cat#I5500 RIPA Buffer Solarbio Cat#R0020 Blue Plus II Protein Marker Transgen Biotech Cat#L50824 High Pure dNTPs Transgen Biotech Cat#20201025 GoTaq Green Master Mix Promega Cat#M7122 M-MLV reverse transcriptase Promega Cat#M1701 Random Primers Promega Cat#C1181 FBS TRINTY TEK Cat#01010102 Collagenase Type2 Worthington Cat#LS004176 SYBRTM Green Mix Roche Cat#4913914001 TriPure Isolation Reagent Roche Cat#94015120 Methanol Fisher Chemical Cat#A452 Water Fisher Chemical Cat#A4 Acetonitrile Fisher Chemical Cat#A998 Ammonium acetate Fisher Chemical Cat#A114 Ammonium hydroxide Fisher Chemical Cat#470 MG132 Sigma Cat#474787 leupetin Sigma Cat# L2884 Software Graphpad Graphpad softwave Graphpad.com ..
Reverse Transcription:Article Title: WTAP regulates postnatal development of brown adipose tissue by stabilizing METTL3 in mice
Article Snippet: .. Data represent the mean ± SEM. n was the number of biologically independent mice. a b W ta p flo x/ flo x W ta pB K O W ta pB K O M et tl3 -B O E 0 2 4 6 F o o d i n ta k e ( g /m o u s e /d a y ) D ar k cy cl e Li gh t c yc le 0 50 100 150 200 250 P h y s ic a l a c ti v it y ( m ) Wtap flox/flox Wtap-BKO Wtap-BKO Mettl3-BOE ** * Wtap flox/flox Wtap-BKO Wtap-BKO Mettl3-BOE c 100um Supplementary Table 3 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies METTL3 CST Cat#96391 WTAP proteintech Cat# 10200-1-AP UCP1 SIGMA Cat#U6382 PGC-1α proteintech Cat#66369-1-lg β-Actin proteintech Cat#60008-1-lg PPARγ proteintech Cat#16643-1-AP PRDM16 Abclonal Cat# A11581 Total OXPHOS abcom Cat#ab110413 GAPDH proteintech Cat#60004-1-lg Flag SIGMA Cat# F1804 AKT CST Cat#9272 P-AKT(S473) CST Cat#9271 Chemicals IBMX(isobutylmethylx anthine) SIGMA Cat#I5879 T3 SIGMA Cat#T2877 ROSIGLITAZONE SIGMA Cat#R2408 INDOMETHACIN CRYSTALLINE SIGMA Cat#I7378 DEXAMETHASINE CRYSTALLINE SIGMA Cat#D1756 INSULIN SIGMA Cat#I5500 RIPA Buffer Solarbio Cat#R0020 Blue Plus II Protein Marker Transgen Biotech Cat#L50824 High Pure dNTPs Transgen Biotech Cat#20201025 GoTaq Green Master Mix Promega Cat#M7122 M-MLV reverse transcriptase Promega Cat#M1701 Random Primers Promega Cat#C1181 FBS TRINTY TEK Cat#01010102 Collagenase Type2 Worthington Cat#LS004176 SYBRTM Green Mix Roche Cat#4913914001 TriPure Isolation Reagent Roche Cat#94015120 Methanol Fisher Chemical Cat#A452 Water Fisher Chemical Cat#A4 Acetonitrile Fisher Chemical Cat#A998 Ammonium acetate Fisher Chemical Cat#A114 Ammonium hydroxide Fisher Chemical Cat#470 MG132 Sigma Cat#474787 leupetin Sigma Cat# L2884 Software Graphpad Graphpad softwave Graphpad.com ..
Isolation:Article Title: WTAP regulates postnatal development of brown adipose tissue by stabilizing METTL3 in mice
Article Snippet: .. Data represent the mean ± SEM. n was the number of biologically independent mice. a b W ta p flo x/ flo x W ta pB K O W ta pB K O M et tl3 -B O E 0 2 4 6 F o o d i n ta k e ( g /m o u s e /d a y ) D ar k cy cl e Li gh t c yc le 0 50 100 150 200 250 P h y s ic a l a c ti v it y ( m ) Wtap flox/flox Wtap-BKO Wtap-BKO Mettl3-BOE ** * Wtap flox/flox Wtap-BKO Wtap-BKO Mettl3-BOE c 100um Supplementary Table 3 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies METTL3 CST Cat#96391 WTAP proteintech Cat# 10200-1-AP UCP1 SIGMA Cat#U6382 PGC-1α proteintech Cat#66369-1-lg β-Actin proteintech Cat#60008-1-lg PPARγ proteintech Cat#16643-1-AP PRDM16 Abclonal Cat# A11581 Total OXPHOS abcom Cat#ab110413 GAPDH proteintech Cat#60004-1-lg Flag SIGMA Cat# F1804 AKT CST Cat#9272 P-AKT(S473) CST Cat#9271 Chemicals IBMX(isobutylmethylx anthine) SIGMA Cat#I5879 T3 SIGMA Cat#T2877 ROSIGLITAZONE SIGMA Cat#R2408 INDOMETHACIN CRYSTALLINE SIGMA Cat#I7378 DEXAMETHASINE CRYSTALLINE SIGMA Cat#D1756 INSULIN SIGMA Cat#I5500 RIPA Buffer Solarbio Cat#R0020 Blue Plus II Protein Marker Transgen Biotech Cat#L50824 High Pure dNTPs Transgen Biotech Cat#20201025 GoTaq Green Master Mix Promega Cat#M7122 M-MLV reverse transcriptase Promega Cat#M1701 Random Primers Promega Cat#C1181 FBS TRINTY TEK Cat#01010102 Collagenase Type2 Worthington Cat#LS004176 SYBRTM Green Mix Roche Cat#4913914001 TriPure Isolation Reagent Roche Cat#94015120 Methanol Fisher Chemical Cat#A452 Water Fisher Chemical Cat#A4 Acetonitrile Fisher Chemical Cat#A998 Ammonium acetate Fisher Chemical Cat#A114 Ammonium hydroxide Fisher Chemical Cat#470 MG132 Sigma Cat#474787 leupetin Sigma Cat# L2884 Software Graphpad Graphpad softwave Graphpad.com ..
Software:Article Title: WTAP regulates postnatal development of brown adipose tissue by stabilizing METTL3 in mice
Article Snippet: .. Data represent the mean ± SEM. n was the number of biologically independent mice. a b W ta p flo x/ flo x W ta pB K O W ta pB K O M et tl3 -B O E 0 2 4 6 F o o d i n ta k e ( g /m o u s e /d a y ) D ar k cy cl e Li gh t c yc le 0 50 100 150 200 250 P h y s ic a l a c ti v it y ( m ) Wtap flox/flox Wtap-BKO Wtap-BKO Mettl3-BOE ** * Wtap flox/flox Wtap-BKO Wtap-BKO Mettl3-BOE c 100um Supplementary Table 3 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies METTL3 CST Cat#96391 WTAP proteintech Cat# 10200-1-AP UCP1 SIGMA Cat#U6382 PGC-1α proteintech Cat#66369-1-lg β-Actin proteintech Cat#60008-1-lg PPARγ proteintech Cat#16643-1-AP PRDM16 Abclonal Cat# A11581 Total OXPHOS abcom Cat#ab110413 GAPDH proteintech Cat#60004-1-lg Flag SIGMA Cat# F1804 AKT CST Cat#9272 P-AKT(S473) CST Cat#9271 Chemicals IBMX(isobutylmethylx anthine) SIGMA Cat#I5879 T3 SIGMA Cat#T2877 ROSIGLITAZONE SIGMA Cat#R2408 INDOMETHACIN CRYSTALLINE SIGMA Cat#I7378 DEXAMETHASINE CRYSTALLINE SIGMA Cat#D1756 INSULIN SIGMA Cat#I5500 RIPA Buffer Solarbio Cat#R0020 Blue Plus II Protein Marker Transgen Biotech Cat#L50824 High Pure dNTPs Transgen Biotech Cat#20201025 GoTaq Green Master Mix Promega Cat#M7122 M-MLV reverse transcriptase Promega Cat#M1701 Random Primers Promega Cat#C1181 FBS TRINTY TEK Cat#01010102 Collagenase Type2 Worthington Cat#LS004176 SYBRTM Green Mix Roche Cat#4913914001 TriPure Isolation Reagent Roche Cat#94015120 Methanol Fisher Chemical Cat#A452 Water Fisher Chemical Cat#A4 Acetonitrile Fisher Chemical Cat#A998 Ammonium acetate Fisher Chemical Cat#A114 Ammonium hydroxide Fisher Chemical Cat#470 MG132 Sigma Cat#474787 leupetin Sigma Cat# L2884 Software Graphpad Graphpad softwave Graphpad.com ..
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